UHPLC Column, RP PFP, 1.8um, 100A. 2.1mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68122‑10P‑2 Cogent RP PFP™ UHPLC Column is designed for enhanced selectivity of halogenated, aromatic, and polar compounds, offering improved resolution where traditional C18 phases may not fully differentiate analytes.
This stationary phase features a pentafluorophenyl (PFP) ligand bonded via a four‑carbon linker to high‑purity silica with full double end‑capping, enabling multiple interaction mechanisms such as π‑π interactions, dipole interactions, and hydrogen bonding. This combination provides greater selectivity control and improved separation of structurally similar compounds.
The phase is ideal for USP L43 methods and for method development where alternative selectivity is required.
100 mm Length – Increased Resolution for LC‑MS Methods
The 100 mm length provides greater resolving power than 50 mm columns, allowing improved separation while maintaining efficient run times.
Better resolution of closely related compounds
Increased peak capacity for complex samples
Enhanced selectivity for difficult separations
✅ Use when 50 mm columns do not provide sufficient resolution
Flow Rate & System Requirements (2.1 mm ID, 1.8 µm)
⚠️ Requires UHPLC system capability due to high backpressure
Typical flow rate range: ~0.2–0.4 mL/min
Optimal starting range: ~0.25–0.35 mL/min
Requires precise, stable low‑flow pumping
Compatible with low‑volume LC‑MS detectors
✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection
Why Choose PFP for Selective Separations
Enhanced Retention for Halogenated Compounds
Strong interaction with fluorinated and halogenated analytes
Improved selectivity vs C18
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding effects
Greater flexibility in method development
Improved Resolution Without Excessive Run Time
Higher selectivity enables better separation efficiency
Useful for optimizing existing LC‑MS methods
Cogent RP PFP™ Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry for polar compounds
High Efficiency (1.8 µm Particles)
Increased plate counts and peak capacity
Faster, high‑resolution separations
High Purity Silica
Consistent column‑to‑column performance
Reliable reproducibility across analytical methods
Typical Applications
Halogenated compound analysis
Aromatic compound separations
Pharmaceutical method development
LC‑MS analytical workflows
USP L43 method applications
Important Usage Notes
⚠️ Requires UHPLC system (high pressure capable)
Designed for low‑flow LC‑MS workflows
Higher backpressure than larger particle columns
Use 0.2 µm filtration to maintain column performance